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EPO offers different
routes of stem cell transplantation for testing the tumourigenic
potential.
The tumourigenic potential of pluripotent stem cell preparations are
evaluated either in the syngeneic immunocompetent mouse strain
S129/SVE mice (murine embryonic stem cells) or immunodeficient
NOD/SCID mice model (human embryonic stem cells*).
For special purposes different routes of transplantation of stem
cell preparations can be performed.
Evaluation: Histological and immunohistochemical evaluation for
formation of different germ layer structures (mesoderm, endoderm,
ectoderm) as well as for evidence of teratoma and teratocarcinoma
formation in different organs.
The organ distribution of embryonic stem cells (ESC) can be
investigated using fluorescence detection of EYFP labelled R1 mouse
ES cells in different mouse organs by FACS analysis and
immunohistochemistry (murine ESC) or can be determined by human
specific-PCR method (human ESC).
* only with the approval of the responsible
authorities |
Teratoma formation after
subcutaneous application of undifferentiated murine embryonic stem
cells in S129/SVE mice. HE staining of the teratoma. The teratoma
consists of differentiated tissue from all three germ layers.
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