Immunohistochemistry (IHC) and immunofluorescence can be performed with in vitro or in vivo samples.
In vitro the cells are grown on cell culture slides suitable for IHC or are spinned onto the glass slides (cytospin).
The in vivo tumour samples are sectioned with a Leica cryostat CM at -20°C. The sections can be analysed after Haematoxilin-Eosin-staining or after specific antibody staining, using either peroxidase or fluorescent techniques.
 

With optimised staining protocols and a broad range of antibodies we are able to detect proteins of the cytoskeleton (e.g. vimentin, EpCAM, cytokeratin, tubulin), proliferation markers (Ki67), numerous receptors (EGFR, viscumin) and cells of the immunsystem as well as many other proteins. Examples of our panel are given below.