 |
|
HEK293 cells expressing luciferase activity; magnification: 200x |
 |
| Bioluminescence image from NMRI-nude mouse HEK293 cells transfected with the pEGFPLuc-vector were applied subcutaneously. Image was taken 41 days after cell injection. |
Luciferase is an enzyme that emits light when supplied with one of its
substrates, e.g. luciferin. This bioluminescence is used for several molecular
approaches and the advantages are the fast and easy in vitro and in vivo
detection, the high sensitivity as well as its non hazardous and non radioactive
properties.
The plasmid pEGFPLuc (Clontech) expressing the green fluorescence protein (GFP)
and the luciferase gene from firefly Photinus pyralis was stably transfected
into different cell types. The determination of GFP is performed by flow
cytometric analysis and luciferase activity using the luciferase assay and
GloMax luminometer device from Promega.
Cells transfected with this plasmid can be used for non-invasive in vivo imaging
to monitor engraftment or metastasis of different cell types. An appropriate
device (VisLuxxII, Visitron Systems) is available. Further on, pEGFPLuc can be
used as comarker to investigate transfection efficiencies.
|
|
|
HEK293 cells expressing luciferase activity; magnification: 200x |
|
|
| Bioluminescence image from NMRI-nude mouse HEK293 cells transfected with the pEGFPLuc-vector were applied subcutaneously. Image was taken 41 days after cell injection. |
Copyright © 2007 of EPO GmbH - All rights reserved. 04. März 2008.
Top