Cytotoxicity testing: Growth inhibition (MTT assay)
The MTT assay is a rapid and convenient non-radioactive standard-method to measure cell viability. The metabolic activity of cells is determined by adding the yellow tetrazolium salt (MTT), which is mitochondrially reduced to insoluble purple formazan by viable cells. This colorimetric assay is suitable for drug sensitivity or cytotoxicity test and is in our laboratory adapted for adherent as well as suspension cells.
Protocol: Cells are seeded in appropriate number into 96-well
plates and treated with different concentrations compounds 24 hours after
seeding. After 4-day-incubation, MTT salt is added and formazan measured in a
plate reader at 540 nm. The IC50 values are calculated from the response curves
[Fig. 1].
Fig. 1: MTT assay to determine the sensitivity of breast carcinoma cells to doxorubicin. Assay is performed 3-fold.
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