Immuno modulation

Drug effects mediated by the immune system are evaluated by quantification of secreted soluble factors in mouse serum/plasma or by measuring phenotypical or function-related parameters of mouse immune cells.

Changes in blood / serum parameters:

Number of white blood cells, platelets and erythrocytes; Haematokrit (HCT), Haemaglobin (HGB); Mean corpuscular volume (MCV), corpuscular haemoglobin (MCH) and corpuscular haemoglobin concentration (MCHC) are quantified by coulter counter measurements
Cytokines, growth factors or immuno modulators are quantified in mouse serum or plasma using ELISA kits or antibodies
Number of leucocytes and subsets of mouse T-, B and NK cells are determined by FACS analysis
Differentiation of white blood cells and granulotopoesis is performed by microscopy

Characterisation of mouse immune cells:

T-, B-, NK- and monocytic/dendritic cells are isolated from blood or spleen using Magnetic Cell Separation (Miltenyi). Purity control of isolated immune cells is performed by FACS analysis. Functional parameters (basal and stimulation capacity, intracellular level of IL-2, TNFα IFNγ or other cytokines) are measured by FACS analysis.

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